Design plasmid maps
Design your plasmids
Sequence v isualization of circular and linear DNA
Customize view : preferred set of enzymes, features, primers for PCR and sequencing, ORFs
Import your plasmid in .gb format or copy/paste your DNA sequence
Adapt origin, edit sequence (deletions, additions, substitutions) without errors
Use automatic annotations : specific sequences are automatically recognized as plasmid features
Link plasmids to project, and all derived products such as plasmid preps, glycerols stocks, cells, proteins, aliquots, etc.
Verify cloning process and avoid errors
Cloning processes can be simulated to avoid errors . Inserts may come from plasmid restriction, DNA synthesis, or PCR amplification.
Available cloning options: classical ligation, infusion, yeast recombination, Golden Gate.
Automatic creation of the new plasmid molecule.
Create a preferred sets of enzymes that can be used for several plasmids.
Keep history of your constructs
Track samples and Analyse DNA sequence
Analyze sequencing data
Gather all your primers in the same tab and automatically find information such as melting temperature, GC content, length, molecular weight, and more.
Find in one click if you already have adequate primers in your lab. Yu-Clone helps you in the design of additional primers.
Compare sequencing results to theoretical plasmid. Yu-Clone helps in the identification of mismatches and enables manual correction if necessary.
Automatic filling of 'status of analysis' , depending on sequencing results (invalidated, partial, validated)
Easy synthetic biology
MoClo (Modular Cloning)
Yu-Golden Gate helps you to work with MoClo toolkit standards, such as the classic MoClo tool kit, CIDAR MoClo parts, MoClo yeast tool kit, MoClo Plants tool kit and MoClo Pichia tool kit. It also enables you to design your own standard, with the personalization of fusion sites and assembly models. Consistency of your standard is verified automatically.
Automatic identification of Golden Gate parts in your plasmid molecules
Get in one click all the parts compatible with specific fusion sites and type-IIS restriction enzymes.
Get in one click all the compatible acceptor vectors.
Combinatorial in-silico cloning : Yu-Golden Gate verifies and creates all the plasmids molecules you desire in few clicks.
Calculated volumes of each parts are ready to be sent to your favorite pipetting robot.